Description
[Procedure]
Note: Testing must be conducted at ≥20°C. For frozen raw milk with visible particles, heat or centrifuge the sample and test the middle layer to avoid incomplete flow.
- Tear open the β-lactam antibiotic test strip package, remove the test card, and place it flat on a table. Remove the gold-labeled micro-well from its foil pouch.
- Use a pipette to transfer 120 μL of raw milk (or 4–5 drops with a dropper) into the gold-labeled micro-well. After 2 minutes, mix the solution thoroughly with the dropper.Wait another 2 minutes, then transfer all the mixture to the sample well on the test card using the dropper.
- Lay the card flat and read results after 10 minutes. Results are invalid after 30 minutes.

[Result Interpretation]
- Negative (–): Two purple bands. T line ≥ C line . This indicating β-lactam antibiotic levels below the detection lineor no residue.
- Positive (+): T line < C line or absent. This Indicating β-lactam antibiotic levels above the detection line. A fainter T line corresponds to higher residue concentration.
- Invalid: No C line. This indicates improper operation or an expired test card.Re-read the manual and retest with a new card. If issues persist, discontinue use and contact the supplier.
[Product performance]
- [Detection Limits]
The table below lists the detection limits (ppb) for some β-lactam antibiotics:
| Antibiotic | cut-off | Antibiotic | cut-off |
| Penicillin G | 4 ppb | Amoxicillin | 6 ppb |
| Ampicillin | 6 ppb | Ceftiofur and metabolites | 60 ppb |
| Cephapirin | 15 ppb |

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